改良融合技术。I. 人脐带血清，一种新型强效生长促进剂，与其他B细胞和杂交瘤激活剂的比较。
Improved fusion technique. I. Human umbilical cord serum, a new and potent growth promoter, compared with other B cell and hybridoma activators.
作者信息
Westerwoudt R J, Blom J, Naipal A M, Van Rood J J
出版信息
J Immunol Methods. 1983 Aug 12;62(1):59-67. doi: 10.1016/0022-1759(83)90110-2.
Accelerated proliferation of hybridoma cells was observed in the presence of human umbilical cord serum (HUCS). This had very strong growth-promoting activity, even at a concentration of 2%. A comparison was made between HUCS and other B cell growth promoters, such as lipopolysaccharide (LPS) and dextran sulfate (DxS), macrophage supernatant, and human endothelial culture supernatant (HECS). The growth-promoting effect of HUCS was superior. Using a microcytotoxicity assay, we found no significant differences in the number of antibody producing clones with the various culture media, except for fetal calf serum.
在人脐带血清（HUCS）存在的情况下，观察到杂交瘤细胞的增殖加速。即使在浓度为2%时，它也具有很强的促生长活性。对HUCS与其他B细胞生长促进剂进行了比较，如脂多糖（LPS）、硫酸葡聚糖（DxS）、巨噬细胞上清液和人内皮细胞培养上清液（HECS）。HUCS的促生长作用更优。使用微量细胞毒性试验，我们发现除胎牛血清外，在各种培养基中产生抗体的克隆数量没有显著差异。
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