Ohyama H, Shimizu M, Yamada T
Radiat Res. 1983 Jul;95(1):116-23.
The effect of sodium bisulfite, a specific inhibitor of chromatin proteolysis, on radiation damage in rat thymocytes in vitro was examined. Rat thymocytes irradiated with 1 kR X rays in vitro were incubated at 37 degrees C with 10 mM glucose for 4 to 6 hr. During that time development of interphase death as judged by erythrosin B uptake, release of low molecular weight DNA (free DNA), and reduction in cell size was measured. Sodium bisulfite added to the cells at the beginning of incubation exerted a marked preventive effect on radiation damage. The effect was enhanced with increasing concentration of bisulfite from 0.25 to 2 mM. The effect of bisulfite was reversible; i.e., removal of bisulfite from the cells resulted in the reappearance of the radiation damage.
研究了亚硫酸氢钠（一种染色质蛋白水解的特异性抑制剂）对体外培养的大鼠胸腺细胞辐射损伤的影响。体外经1kR X射线照射的大鼠胸腺细胞于37℃与10mM葡萄糖孵育4至6小时。在此期间，通过伊红B摄取、低分子量DNA（游离DNA）释放和细胞大小减小来判断间期死亡的发展情况。在孵育开始时添加到细胞中的亚硫酸氢钠对辐射损伤具有显著的预防作用。随着亚硫酸氢钠浓度从0.25mM增加到2mM，这种作用增强。亚硫酸氢钠的作用是可逆的；即，从细胞中去除亚硫酸氢钠会导致辐射损伤再次出现。
