Shvedova A A, Kagan V E, Kuliev I Ia, Vekshina O M
Biull Eksp Biol Med. 1983 Aug;96(8):48-50.
Mechanisms of the photo-damage of a fluorescent dye (methylene blue) and of the protective action of antioxidants and quenchers of singlet oxygen on the outer retinal rod segments (ORRS) and retinal function in situ and in vivo were studied. The methylene blue-induced formation of singlet oxygen in the ORRS resulted in accumulation of lipid peroxidation products, oligomerization of rhodopsin, and in a decrease in rhodopsin thermal resistance. Modification of the lipid and protein components of the visual cells by singlet oxygen inhibited the electrical activity of both isolated frog retina in situ and rabbit retina in vivo (waves a and b on the electroretinogram). The antioxidants (alpha-naphthol, alpha-tocopherol, 4-methyl-2,6-di-tert-butylphenol) and singlet oxygen quenchers, [1,4-diazabicyclo (2,2,2)octane] and alpha-tocopherol prevented the damaging effects of the fluorescent dye induced by singlet oxygen formation.
研究了荧光染料（亚甲蓝）的光损伤机制，以及抗氧化剂和单线态氧猝灭剂对视网膜外段视杆细胞（ORRS）的保护作用，以及在原位和体内对视网膜功能的影响。亚甲蓝在ORRS中诱导单线态氧的形成，导致脂质过氧化产物的积累、视紫红质的寡聚化以及视紫红质热稳定性的降低。单线态氧对视细胞脂质和蛋白质成分的修饰抑制了原位分离的青蛙视网膜和体内兔子视网膜的电活动（视网膜电图上的a波和b波）。抗氧化剂（α-萘酚、α-生育酚、4-甲基-2,6-二叔丁基苯酚）和单线态氧猝灭剂[1,4-二氮杂双环（2,2,2）辛烷]以及α-生育酚可防止由单线态氧形成引起的荧光染料的损伤作用。
