Cook R G, Landolfi N F
J Exp Med. 1983 Sep 1;158(3):1012-7. doi: 10.1084/jem.158.3.1012.
Peripheral T lymphocytes activated in vitro with concanavalin A (Con A) or alloantigens express the thymus leukemia (TL) alloantigen as assessed by staining with the monoclonal antibody TL.m3 and flow cytometric analysis. The determinants detected by TL.m3 on activated cells are encoded within the Tla region and are detected as early as 48 h after activation with Con A. Several long-term cloned cytotoxic T lymphocyte lines were also examined and each expressed TL. By two-dimensional analysis, the TL isolated from activated peripheral cells was indistinguishable from that found on thymocytes and the leukemia cell line ASL-1.
用刀豆球蛋白A(Con A)或同种异体抗原在体外激活的外周T淋巴细胞,通过用单克隆抗体TL.m3染色和流式细胞术分析评估,表达胸腺白血病(TL)同种异体抗原。TL.m3在活化细胞上检测到的决定簇在Tla区域内编码,并且在用Con A激活后最早在48小时即可检测到。还检查了几个长期克隆的细胞毒性T淋巴细胞系,每个都表达TL。通过二维分析,从活化外周细胞中分离的TL与在胸腺细胞和白血病细胞系ASL-1上发现的TL无法区分。