Schlunk T, Schleyer M
Blut. 1983 Oct;47(4):211-23. doi: 10.1007/BF00320840.
For preparative purification of colony-stimulating factors (CSF) from human placental conditioned medium, cation exchange chromatography, gel filtration, and isoelectric focusing were combined. The individual fractions were tested using the growth in agar of cryopreserved human bone marrow (dose-response curves). Furthermore, the active preparations were characterized by analytical isoelectric focusing. Two fractions with highly enriched biological activity were isolated. They have the isoelectric points 5.4 and 5.6, respectively. Their specific activity exceeds that of the crude material by a factor of 60. Like crude material, they induce predominantly granulocytic colonies. The biochemical heterogeneity of biological activity in placental supernatants is discussed.
为了从人胎盘条件培养基中制备纯化集落刺激因子（CSF），将阳离子交换色谱、凝胶过滤和等电聚焦结合起来。使用冻存人骨髓在琼脂中的生长情况（剂量-反应曲线）对各个组分进行检测。此外，通过分析性等电聚焦对活性制剂进行表征。分离出了两个具有高度富集生物活性的组分。它们的等电点分别为5.4和5.6。它们的比活性比粗制品高出60倍。与粗制品一样，它们主要诱导粒细胞集落。讨论了胎盘上清液中生物活性的生化异质性。
