Wu M C, Yunis A A
J Clin Invest. 1980 Mar;65(3):772-5. doi: 10.1172/JCI109726.
Conditioned media prepared from human lung, placenta, peripheral leukocytes, cultured human pancreatic carcinoma cells, and cultured cervical carcinoma cells exhibit a common pattern of two distinct colony-stimulating factors (CSF) separable by isoelectrofocusing. Type I CSF appears polydisperse on isoelectrofocusing, showing multiple peaks with an isoelectric point of 3.6-4.7 and mol wt 50,000. It has a much higher activity in mouse than in human bone marrow, and stimulates the formation of predominately macrophage colonies. Type II CSF has an isoelectric point of 5.7 and mol wt 27,000 and exhibits higher activity in human than in mouse marrow, yielding predominately granulocytic colonies.
从人肺、胎盘、外周血白细胞、培养的人胰腺癌细胞和培养的宫颈癌细胞制备的条件培养基表现出一种常见模式,即通过等电聚焦可分离出两种不同的集落刺激因子(CSF)。I型CSF在等电聚焦上呈多分散性,显示出多个峰,其等电点为3.6 - 4.7,分子量为50,000。它在小鼠骨髓中的活性比在人骨髓中高得多,并主要刺激巨噬细胞集落的形成。II型CSF的等电点为5.7,分子量为27,000,在人骨髓中的活性比在小鼠骨髓中高,主要产生粒细胞集落。