白血病人类T细胞的集落形成及白细胞介素2的产生
Colony formation and interleukin 2 production by leukaemic human T cells.
作者信息
Krajewski A S, Dewar A E, Seidelin P H, Murray R
出版信息
Clin Exp Immunol. 1983 Oct;54(1):103-9.
PHA-induced colony formation and interleukin 2 (IL-2) production were studied in four patients with T cell leukaemia (three cases OKT4+/T helper and one case OKT8+/T cytotoxic suppressor). Cases of T helper cell leukaemia showed colony formation that was comparable to normal purified blood T cells and was not dependent on the addition of conditioned medium, containing IL-2 activity, to cultures. In contrast the T suppressor cell leukaemia formed colonies only when cultures were supplemented with IL-2 containing medium. When IL-2 production by PHA stimulated cells was measured culture supernatants from the three T helper cell leukaemias all showed normal or high levels of activity, when compared to normal blood mononuclear cells, whereas the T suppressor cell leukaemia showed no activity.
对4例T细胞白血病患者（3例OKT4 + / T辅助细胞型和1例OKT8 + / T细胞毒性抑制细胞型）进行了PHA诱导的集落形成和白细胞介素2（IL-2）产生的研究。T辅助细胞白血病病例显示出与正常纯化血液T细胞相当的集落形成，并且不依赖于向培养物中添加含有IL-2活性的条件培养基。相反，T抑制细胞白血病仅在培养物补充含IL-2的培养基时才形成集落。当测量PHA刺激细胞产生的IL-2时，与正常血液单核细胞相比，3例T辅助细胞白血病的培养上清液均显示正常或高水平的活性，而T抑制细胞白血病则无活性。
Zotero 插件