Reinherz E L, Kung P C, Breard J M, Goldstein G, Schlossman S F
J Immunol. 1980 Apr;124(4):1883-7.
Lymphocyte mitogenic factor functions as a nonspecific helper molecule generated from antigen stimulation of T lymphocytes. As such, it induces proliferation of all major lymphocyte subclasses (T, B, and Null cells) and B cell immunoglobulin synthesis. In the present study, the specific T cell subset requirements for LMF production were determined in man. By utilizing the OKT4 monoclonal antibody directed at the human inducer T cell subset, T lymphocytes were separated into OKT4+ and OKT4- subpopulations. The antigen stimulated OKT4+ subset generated LMF in a fashion comparable to the unfractionated T cell population and was itself capable of directly inducing B cell proliferation and antibody production. In contrast, LMF could not be generated from the OKT4-T cell subset although this population accounted for 40% of the unfractionated T cell population. In addition, the OKT4- subset was unable to facilitate B cell differentiation in the presence of soluble antigen. These studies provide the first report of T cell subset restriction for generation of helper factors in man and further stress the importance of the OKT4+ inducer population in regulation of the human immune response.
淋巴细胞促有丝分裂因子作为一种由T淋巴细胞抗原刺激产生的非特异性辅助分子发挥作用。因此，它可诱导所有主要淋巴细胞亚类（T细胞、B细胞和裸细胞）增殖以及B细胞免疫球蛋白合成。在本研究中，确定了人类产生淋巴细胞促有丝分裂因子所需的特定T细胞亚群。通过利用针对人类诱导性T细胞亚群的OKT4单克隆抗体，将T淋巴细胞分离为OKT4 +和OKT4 -亚群。抗原刺激的OKT4 +亚群产生淋巴细胞促有丝分裂因子的方式与未分离的T细胞群体相当，并且其自身能够直接诱导B细胞增殖和抗体产生。相比之下，尽管OKT4 - T细胞亚群占未分离T细胞群体的40%，但无法从该亚群产生淋巴细胞促有丝分裂因子。此外，在可溶性抗原存在的情况下，OKT4 -亚群无法促进B细胞分化。这些研究首次报道了人类产生辅助因子存在T细胞亚群限制，并进一步强调了OKT4 +诱导群体在调节人类免疫反应中的重要性。
