Zeevi A, Annen K, Scheffel C, Duquesnoy R J
Immunogenetics. 1983;18(3):205-20. doi: 10.1007/BF00952960.
This study deals with alloreactive T-cell clones which recognize cellular determinants associated with HLA-DR antigens. Two clones, CB55 and DS56, exhibited a PLT specificity that was perfectly associated with DR5. On the other hand, clones CB7, DS1 and HS1 showed PLT reactivity with approximately one-half of the DR5 positive cells and none of the DR5 negative cells, whereas clone MD4 largely reacted with the other half of DR5 positive cells. Another MLR culture generated two alloreactive clones DS6 and DS9 with PLT specificity for DR2. However, these clones did not respond to DR2 cells, which were also positive for the DR2-associated HLA-B7 and B18 antigens. Monoclonal antibody (mAb) inhibition studies showed heterogenous patterns, whereby monomorphic non-DR mAbs inhibited the DR2-associated PLT clones while the DR5-associated PLT clones were inhibited by different groups of anti-DR and non-DR mAbs. These observations suggest the existence of several lymphocyte-activating determinants associated with HLA-DR antigens. This diversity may be an important consideration in studies of the role of HLA-DR in immune mechanisms and transplant compatibility.
本研究涉及识别与HLA - DR抗原相关的细胞决定簇的同种反应性T细胞克隆。两个克隆CB55和DS56表现出与DR5完全相关的血小板特异性。另一方面，克隆CB7、DS1和HS1对大约一半的DR5阳性细胞有血小板反应性，而对DR5阴性细胞无反应，而克隆MD4主要与另一半DR5阳性细胞反应。另一种混合淋巴细胞培养产生了两个对DR2有血小板特异性的同种反应性克隆DS6和DS9。然而，这些克隆对DR2细胞无反应，DR2细胞同时也对与DR2相关的HLA - B7和B18抗原呈阳性。单克隆抗体（mAb）抑制研究显示出异质性模式，即单态性非DR mAb抑制与DR2相关的血小板克隆，而与DR5相关的血小板克隆被不同组的抗DR和非DR mAb抑制。这些观察结果表明存在几种与HLA - DR抗原相关的淋巴细胞激活决定簇。这种多样性可能是研究HLA - DR在免疫机制和移植相容性中的作用时的一个重要考虑因素。
