Cavallo T, Graves K, Granholm N A
Lab Invest. 1983 Oct;49(4):476-81.
We investigated the effects of methylprednisolone on immunoreactants of plasma and kidney to determine factors that might be relevant to the arrest of murine lupus nephritis. At the onset of nephritis, at about 5 months of age, the mice were divided in two groups and received either methylprednisolone or saline injections for 12 weeks. Before and after therapy (or saline injections), we determined the concentrations of plasma IgG, complement (C3), anti-DNA antibodies, Clq-reactive materials, creatinine, and urea nitrogen; in the kidneys, we assessed the relative distribution of IgG, IgM, and C3 in glomeruli, and we determined the concentration of IgG and anti-DNA activity of the eluted proteins. Our results indicated that methylprednisolone administered at the onset of nephritis preserved glomerular structure and function by decreasing the amount of tissue-bound immunoreactants and by inducing a preferential localization of immunoreactants in mesangia. Of the immunoreactants studied in plasma, a decreased concentration of IgG, but not the concentrations of anti-DNA antibodies, C3, and Clq-reactive materials, was associated with the arrest of nephritis. The anti-DNA activity in the renal eluates was very low and comparable in treated and untreated mice. Immune complex systems other than, or in addition to, DNA-anti-DNA likely play a role in the pathogenesis of murine lupus nephritis.
我们研究了甲基强的松龙对血浆和肾脏免疫反应物的影响，以确定可能与小鼠狼疮性肾炎缓解相关的因素。在肾炎发病时，约5月龄时，将小鼠分为两组，分别接受甲基强的松龙或生理盐水注射，为期12周。在治疗（或注射生理盐水）前后，我们测定了血浆IgG、补体（C3）、抗DNA抗体、Clq反应性物质、肌酐和尿素氮的浓度；在肾脏中，我们评估了IgG、IgM和C3在肾小球中的相对分布，并测定了洗脱蛋白的IgG浓度和抗DNA活性。我们的结果表明，在肾炎发病时给予甲基强的松龙可通过减少组织结合免疫反应物的量并诱导免疫反应物在系膜中的优先定位来保留肾小球结构和功能。在血浆中研究的免疫反应物中，IgG浓度降低与肾炎缓解相关，但抗DNA抗体、C3和Clq反应性物质的浓度未降低。肾洗脱物中的抗DNA活性非常低，在治疗和未治疗的小鼠中相当。除DNA-抗DNA之外或之外的免疫复合物系统可能在小鼠狼疮性肾炎的发病机制中起作用。
