Oehmichen M, Kömpf J
Z Rechtsmed. 1983;90(2):127-36. doi: 10.1007/BF02092297.
Blood was taken from the femoral vein of 17 autopsied cadavers with different diagnoses and postmortal intervals (10-120 h). The granulocytes and lymphocytes were isolated with routine methods. The cell suspensions were subject to morphologic, enzyme-cytochemical (naphthol AS-D chloroacetate esterase) and electrophoretic (PGM1, PGM3, GOTM, PEPA, MEM, FUCA) investigations. The following results were obtained: Erythrocyte-free cell suspensions are only found during short postmortal intervals; Isolation of pure lymphocytes from cadaver blood using the described method is only possible very early in the postmortal interval (within 10 h); The isolated granulocytes contain an impressively high percentage of eosinophilic granulocytes; The percentage of naphthol AS-D-chloroacetate esterase-positive granulocytes is considerably lower in stored, conserved blood than in blood smears; Identification of the above mentioned enzymes from isolated granulocytes from all cadavers is possible by electrophoresis.
从17具不同诊断和死后间隔时间（10 - 120小时）的尸体解剖后的股静脉采集血液。用常规方法分离粒细胞和淋巴细胞。对细胞悬液进行形态学、酶细胞化学（萘酚AS - D氯乙酸酯酶）和电泳（PGM1、PGM3、GOTM、PEPA、MEM、FUCA）研究。获得以下结果：仅在短死后间隔期间发现无红细胞的细胞悬液；使用所述方法从尸体血液中分离纯淋巴细胞仅在死后间隔的非常早期（10小时内）才有可能；分离出的粒细胞中嗜酸性粒细胞的百分比高得惊人；储存的保存血液中萘酚AS - D - 氯乙酸酯酶阳性粒细胞的百分比比血涂片低得多；通过电泳可以从所有尸体分离出的粒细胞中鉴定上述酶。
