Berg S, Ladiges M L, Ladiges O
Z Rechtsmed. 1981;87(1-2):85-94. doi: 10.1007/BF00201213.
The isozymes of the PGMa1-system can be determined more or less distinctly in stored blood samples up to 1.5 years in approximately half of all samples. However, in several cases shifting of the isoelectric points and splitting can be noticed, especially in the c-bands range and, as a result of this, the primary type PGM1 a1 can simulate a PGM1 a2-a1 type, or more rarely a PGM1 a2 type. By a small cathodic shift of the a-band even PGM1 a3-a2 type can be found under special circumstances. Dried traces may simulate the PGM1 a2-a1 type through the appearance of conversion-bands in the b-range and on definite trace carriers even the PGM1 a4-a1 type may be simulated. The presentation of the Gc-subtypes is nearly always possible parallel to the Tf-subtypes even in alcohol blood samples stored for up to 3 months and in most of those stored for up to 7 months. Later, it often comes to a widening and splitting of the 1-band so that a discrimination of the S- and F-homo- and heterozygotes, respectively, is not possible anymore. Altogether, Gc-basic types may be more easily determined by isoelectric focusing in aged blood samples than with the electrophoretic methods including immunofixation.
在大约一半的样本中，PGMα1系统的同工酶在保存长达1.5年的血样中或多或少能被清晰地检测到。然而，在一些情况下，可以注意到等电点的偏移和条带分裂，尤其是在c带范围内，因此，主要类型PGM1α1可能会模拟PGM1α2 - α1类型，或更罕见地模拟PGM1α2类型。在特殊情况下，通过α带的微小阴极偏移甚至可以发现PGM1α3 - α2类型。干燥痕迹可能会通过b范围内转化带的出现模拟PGM1α2 - α1类型，在特定的痕迹载体上甚至可能模拟PGM1α4 - α1类型。即使在保存长达3个月的酒精血样以及大多数保存长达7个月的血样中，几乎总能与Tf亚型平行地呈现Gc亚型。后来，1带常常会变宽和分裂，以至于分别区分S型和F型纯合子及杂合子不再可能。总体而言，与包括免疫固定在内的电泳方法相比，通过等电聚焦在陈旧血样中可能更容易确定Gc基本类型。
