Penttilä A, Laiho K
Forensic Sci Int. 1981 Mar-Apr;17(2):121-32. doi: 10.1016/0379-0738(81)90004-9.
The morphology of various types of cells in the peripheral blood of human cadavers was investigated. The material comprised 123 medicolegal autopsy cases with post-mortem (p.m.) times ranging fro 1.7 to 270.4 hours. The corpses were kept at +4 degrees C. The haematocrit values of the blood increased rapidly after death. The haematocrit-corrected red cell count, and the total white cell and platelet counts remained quite stable during the whole p.m. time range. Red cells were quite rapidly transformed from a discoid configuration to crumbled discs, echinocytes and spherocytes, but no debris or burst cell configurations were seen. Rapid deterioration of the staining properties and marked morphological changes in many leucocytes occurred quite rapidly after death. Lymphocytes seemed to be the most resistant and basophils the least resistant to the effects of autolysis. Morphologically altered platelets and aggregates of them were seen in each cadaver. The present morphological observations and the quantitative results suggest that various cellular elements of the blood seem to be quite resistant to autolytic effects, and many cells apparently retain their viability for longer periods of time in the blood of cadavers kept at reduced temperature.
对人类尸体外周血中各类细胞的形态进行了研究。材料包括123例法医学尸检案例，死后（p.m.）时间从1.7小时至270.4小时不等。尸体保存在4摄氏度环境下。死后血液的血细胞比容值迅速升高。在整个死后时间范围内，经血细胞比容校正的红细胞计数、白细胞总数和血小板计数保持相当稳定。红细胞很快从盘状形态转变为破碎的圆盘状、棘形红细胞和球形红细胞，但未观察到碎片或破裂细胞形态。死后许多白细胞的染色特性迅速恶化且形态发生明显变化。淋巴细胞似乎对自溶作用最具抵抗力，嗜碱性粒细胞对自溶作用抵抗力最弱。在每具尸体中均可见到形态改变的血小板及其聚集体。目前的形态学观察和定量结果表明，血液中的各种细胞成分似乎对自溶作用具有相当的抵抗力，并且许多细胞在保存在低温下的尸体血液中显然能在较长时间内保持其活力。
