Santangelo F, Montecucchi P C, Gozzini L, Henschen A
Int J Pept Protein Res. 1983 Sep;22(3):348-54. doi: 10.1111/j.1399-3011.1983.tb02101.x.
The solid-phase synthesis of the tetracosapeptide corresponding to the C-terminal amino acid sequence of sauvagine is described. After purification by gel filtration, the polypeptide appeared to possess an acceptable degree of homogeneity, as judged by different kinds of electrophoresis and chromatography, and by automated Edman degradation analysis. Preliminary pharmacological results indicate that the fragment-(17-40) is practically devoid of any sauvagine activity on the circulatory system and endocrine glands; a weak effect on gastric emptying delay has been demonstrated (1% of the natural product).
本文描述了与蛙皮素C端氨基酸序列相对应的二十四肽的固相合成。经凝胶过滤纯化后，通过不同类型的电泳、色谱分析以及自动Edman降解分析判断，该多肽似乎具有可接受的均一性程度。初步药理学结果表明，片段（17 - 40）实际上对循环系统和内分泌腺没有任何蛙皮素活性；已证明其对胃排空延迟有微弱作用（为天然产物的1%）。
