Bibikova M V, Ivanitskaia L P, Nikishina V G, Rybakova A M
Antibiotiki. 1983 Sep;28(9):665-71.
A total of 123 cultures of the genus Micromonospora and 89 cultures of the genus Streptoverticillium were studied for their capacity to produce beta-lactamase inhibitors. It was shown that streptoverticilla were highly resistant to beta-lactam antibiotics. No organisms producing beta-lactamase inhibitors were detected among the cultures of this genus. As for Micromonospora, 30 cultures (24.4 per cent) of this genus produced inhibitors. They were most frequently isolated from orange and brown cultures of Micromonospora. The inhibitors had no antibacterial activity, inhibited beta-lactamases of gram-negative bacteria and did not inhibit exogenic beta-lactamases of staphylococci. Their inhibitory activity was evident only with the use of isolated enzymes. With the use of intact growing cultures of gram-negative bacteria producing the same enzymes (Pseudomonas, Klebsiella, Enterobacter) no inhibitory activity was observed. Though the inhibitors had some features in common, they did not seem to be similar, since they had different spectra with respect to their effect on various beta-lactamases of gram-negative bacteria.
对总共123株小单孢菌属培养物和89株轮状链霉菌属培养物进行了产生β-内酰胺酶抑制剂能力的研究。结果表明，轮状链霉菌对β-内酰胺抗生素具有高度抗性。在该属培养物中未检测到产生β-内酰胺酶抑制剂的菌株。至于小单孢菌属，该属有30株培养物（24.4%）产生抑制剂。它们最常从小单孢菌属的橙色和棕色培养物中分离得到。这些抑制剂没有抗菌活性，能抑制革兰氏阴性菌的β-内酰胺酶，但不抑制葡萄球菌的外源性β-内酰胺酶。只有在使用分离的酶时，它们的抑制活性才明显。使用产生相同酶的完整生长的革兰氏阴性菌培养物（假单胞菌属、克雷伯菌属、肠杆菌属）时，未观察到抑制活性。尽管这些抑制剂有一些共同特征，但它们似乎并不相似，因为它们对革兰氏阴性菌的各种β-内酰胺酶的作用谱不同。
