Codegone M L, Bussolati G, Spinnato M R, Stramignoni A
Basic Appl Histochem. 1983;27(3):177-82.
On sections of formalin fixed paraffin embedded lymph nodes, thymuses and spleens, binding of the lectin from peanut (Arachis hypogaea) was detected on some histiocytes, while the lymphoid cells were negative. After treatment of the sections with neuraminidase, sialic acid covered beta-D-galactosyl (1-3)-N-acetyl-D-galactosamine residues were revealed in a more extensive number of histiocytic cells and on a sub-population of lymphoid cells with the typical distribution of T lineage lymphocytes. The technique here described allowing the identification of subclasses of lymphocytes on paraffin sections might provide new data for the study and the diagnosis of lymph node diseases.
在福尔马林固定石蜡包埋的淋巴结、胸腺和脾脏切片上，检测到花生（落花生）凝集素在一些组织细胞上有结合，而淋巴细胞呈阴性。用神经氨酸酶处理切片后，在更多数量的组织细胞以及具有典型T淋巴细胞谱系分布的淋巴细胞亚群上，发现了唾液酸覆盖的β-D-半乳糖基（1-3）-N-乙酰-D-半乳糖胺残基。这里描述的在石蜡切片上鉴定淋巴细胞亚类的技术，可能为淋巴结疾病的研究和诊断提供新的数据。
