Novogrodsky A, Lotan R, Ravid A, Sharon N
J Immunol. 1975 Nov;115(5):1243-8.
Peanut agglutinin, purified by affinity chromatography, agglutinates lymphocytes from mouse, rat, guinea pig, and man only after their treatment with neuraminidase. However, it stimulates only neuraminidase-treated rat and human cells. A similar number cell surface receptors for peanut agglutinin was found on neuraminidase-treated rat and mouse lymphocytes although the latter cells were not stimulated by the lectin. Galactose specifically inhibited the agglutination and stimulation of lymphocytes by peanut agglutinin. Sequential treatment of lymphocytes with neuraminidase and beta-galactosidase markedly reduced the response of the cells to stimulation by peanut agglutinin, soybean agglutinin, and galactose oxidase. It is suggested that the same galactosyl residue may be the target for the initial step in triggering lymphocytes by the above mentioned mitogens.
通过亲和层析纯化的花生凝集素，仅在小鼠、大鼠、豚鼠和人的淋巴细胞经神经氨酸酶处理后才能使其凝集。然而，它仅刺激经神经氨酸酶处理的大鼠和人细胞。尽管后者细胞不受凝集素刺激，但在经神经氨酸酶处理的大鼠和小鼠淋巴细胞上发现了相似数量的花生凝集素细胞表面受体。半乳糖特异性抑制花生凝集素对淋巴细胞的凝集和刺激作用。淋巴细胞先后用神经氨酸酶和β-半乳糖苷酶处理，显著降低了细胞对花生凝集素、大豆凝集素和半乳糖氧化酶刺激的反应。提示相同的半乳糖基残基可能是上述有丝分裂原触发淋巴细胞初始步骤的作用靶点。
