Braley-Mullen H, Tompson J G, Sharp G C, Kyriakos M
Cell Immunol. 1983 Oct 15;81(2):243-52. doi: 10.1016/0008-8749(83)90232-0.
Lymph node T cells from guinea pigs sensitized in vivo with guinea pig thyroglobulin (GPTG) could transfer experimental autoimmune thyroiditis (EAT) to normal syngeneic recipients after in vitro culture with GPTG or GPTG-pulsed peritoneal exudate cells (PEC). Although EAT effector T cells have been shown previously to be Ia negative at the time of transfer, the addition of specific anti-Ia serum to the cultures inhibited effector cell activation. The inhibitory effect of anti-Ia on effector-T-cell activation was shown to be due to inhibition of the function of antigen-presenting PEC rather than to an effect on the sensitized T cell. Moreover, only Ia-positive PEC could present antigen in this system and Ia matching between the PEC and the T cell was required for effective T-cell activation. GPTG-pulsed Strain 2 (EAT susceptible) and Strain 13 (EAT resistant) PEC could both present antigen to T cells from 2 X 13 F1 guinea pigs although Strain 2 PEC were more effective, suggesting that defective antigen presentation by macrophages may at least partially explain the relative resistance to EAT of Strain 13 guinea pigs. These results indicate that interaction between Ia-positive PEC and sensitized T cells in vitro is necessary for the development of active effector T cells that can transfer EAT.
用豚鼠甲状腺球蛋白（GPTG）在体内致敏的豚鼠淋巴结T细胞，在与GPTG或经GPTG脉冲处理的腹腔渗出细胞（PEC）进行体外培养后，可将实验性自身免疫性甲状腺炎（EAT）转移给同基因正常受体。尽管先前已证明EAT效应T细胞在转移时Ia呈阴性，但向培养物中加入特异性抗Ia血清可抑制效应细胞的活化。抗Ia对效应T细胞活化的抑制作用表明是由于抑制了抗原呈递PEC的功能，而非对致敏T细胞的作用。此外，在该系统中只有Ia阳性的PEC能够呈递抗原，并且PEC与T细胞之间的Ia匹配是有效激活T细胞所必需的。经GPTG脉冲处理的2号品系（易患EAT）和13号品系（对EAT有抗性）的PEC都能将抗原呈递给2×13 F1豚鼠的T细胞，尽管2号品系的PEC更有效，这表明巨噬细胞抗原呈递缺陷可能至少部分解释了13号品系豚鼠对EAT的相对抗性。这些结果表明，体外Ia阳性PEC与致敏T细胞之间的相互作用对于能够转移EAT的活性效应T细胞的发育是必要的。
