Braley-Mullen H, Tompson J G, Sharp G C, Kyriakos M
J Immunol. 1981 Nov;127(5):1767-71.
Relatively low numbers of lymph node cells (LNC) from Strain 2 or Strain 13 guinea pigs sensitized with guinea pig thyroglobulin (GPTG) could transfer experimental autoimmune thyroiditis (EAT) to normal syngeneic recipients after in vitro culture with GPTG. The EAT induced in recipients of cultured LNC was similar in incidence and severity to EAT induced by active immunization with GPTG in complete Freund's adjuvant. The cells that were effective in transferring EAT were shown to be immunoglobulin-negative, nylon wool nonadherent, and Ia-negative. The effector cells were sensitive to irradiation after in vitro activation, indicating that cell proliferation in the recipient is required for development of EAT. Recipient animals often developed moderate to severe lesions of EAT yet none had detectable delayed hypersensitivity to GPTG and the majority also had no detectable anti-GPTG antibody.
用豚鼠甲状腺球蛋白（GPTG）致敏的2型或13型豚鼠的淋巴结细胞（LNC）数量相对较少，在与GPTG进行体外培养后，可将实验性自身免疫性甲状腺炎（EAT）转移给同基因正常受体。培养的LNC受体中诱导的EAT在发病率和严重程度上与在完全弗氏佐剂中用GPTG主动免疫诱导的EAT相似。被证明能有效转移EAT的细胞为免疫球蛋白阴性、不黏附尼龙毛且Ia阴性。效应细胞在体外激活后对辐射敏感，这表明受体中的细胞增殖是EAT发生所必需的。受体动物常出现中度至重度的EAT病变，但无一例对GPTG有可检测到的迟发型超敏反应，且大多数也没有可检测到的抗GPTG抗体。
