Levinson S S, Goldman J
Clin Chem. 1983 Dec;29(12):2082-6.
Using a double-beam spectrophotometer, we investigated the clinical utility of a nephelometric method for assaying immune complexes. The complexes were concentrated from serum by precipitation with polyethylene glycol (PEG) and assayed by reaction with C1q. Testing of more than 100 sera showed a Spearman's rank correlation (p) between the present assay and the C1q-binding assay of 0.57, and 0.39 between the Raji cell assay and the present assay. Clinical sensitivity of the methods was not statistically different (p less than or equal to 0.5). Twenty-four of 30 patients with symptoms of disease showed increased concentrations of immune complexes by the present assay; only one of 38 normal individuals showed an increase. In a longitudinal study, we found that the concentrations of immune complexes paralleled clinical changes, indicating good clinical utility. The use of this assay with single-beam analyzers is limited because of the poor aqueous solubility of the PEG precipitate. Ongoing investigations designed to circumvent this problem are described.
我们使用双光束分光光度计研究了一种比浊法检测免疫复合物的临床实用性。通过用聚乙二醇（PEG）沉淀从血清中浓缩复合物，并通过与C1q反应进行检测。对100多份血清的检测显示，本检测方法与C1q结合检测法之间的Spearman等级相关性（p）为0.57，Raji细胞检测法与本检测方法之间的相关性为0.39。这些方法的临床敏感性在统计学上没有差异（p≤0.5）。30例有疾病症状的患者中有24例通过本检测方法显示免疫复合物浓度升高；38名正常个体中只有1例升高。在一项纵向研究中，我们发现免疫复合物的浓度与临床变化平行，表明其具有良好的临床实用性。由于PEG沉淀物的水溶性较差，该检测方法在单光束分析仪上的应用受到限制。本文描述了旨在解决这一问题的正在进行的研究。
