Levinson S S, Goldman J
Am J Clin Pathol. 1983 Apr;79(4):451-7. doi: 10.1093/ajcp/79.4.451.
Clq precipitins were assayed in a model system by absorption nephelometry. Polyethylene glycol was used to extract immune complexes so that sufficient sensitivity with minimal interference could be achieved. Using a double beam spectrophotometer, we found that the reaction of immune complexes with Clq occurred rapidly and plateaued between 5-20 minutes. The optimal concentration of sodium in the reaction mixture was between 0.155-0.165 M, while chloride and EDTA did not affect the reaction significantly. Standard curves generated from heated aggregated gamma globulin showed a high degree of linearity, between 12.5-400 mg/L. Need for conductivity meters, radioisotopes, and other special equipment was eliminated because highly purified Clq is not required for nephelometric assay, and all necessary parameters can be monitored by flame photometry and electrophoresis, which are available in most clinical laboratories. Day to day reproducibility showed CVs of 5.4, 9.5, and 11.4% at control levels of 210, 160, and 70 mg/ L of immune complexes, respectively. Twelve patients with elevated levels of immune complexes by other methods were differentiated from normals by the present method. Nephelometry may offer a simplified approach for testing immune complexes and may become more widespread in clinical laboratories.
通过吸收比浊法在模型系统中检测Clq沉淀素。使用聚乙二醇提取免疫复合物，以便在干扰最小的情况下实现足够的灵敏度。使用双光束分光光度计，我们发现免疫复合物与Clq的反应迅速，在5 - 20分钟之间达到平稳。反应混合物中钠的最佳浓度在0.155 - 0.165 M之间，而氯和乙二胺四乙酸对反应影响不显著。由加热聚集的γ球蛋白生成的标准曲线在12.5 - 400 mg/L之间显示出高度的线性。由于比浊法检测不需要高纯度的Clq，并且所有必要参数都可以通过火焰光度法和电泳进行监测，而大多数临床实验室都有这些设备，因此无需电导率仪、放射性同位素和其他特殊设备。日常重复性显示，在免疫复合物浓度分别为210、160和70 mg/L的对照水平下，变异系数分别为5.4%、9.5%和11.4%。通过本方法将12例通过其他方法检测免疫复合物水平升高的患者与正常人区分开来。比浊法可能为检测免疫复合物提供一种简化的方法，并可能在临床实验室中更广泛地应用。
