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用单克隆抗体和多克隆抗体对兔细胞进行鉴定。

Characterization of rabbit cells by monoclonal and polyclonal antibodies.

作者信息

Ponsard D C, Cinader B, Chou C T, Dubiski S

出版信息

Immunology. 1986 Sep;59(1):115-22.

PMID:3489667
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1453137/
Abstract

Reagents for the identification of rabbit cell markers have been developed at a relatively slow rate. In this paper, rabbit cells are being characterized by polyclonal antibodies against a T-cell antigen (RTLA), a B-cell antigen (RABELA) and an analogue of murine Ia antigen. A number of monoclonal antibodies, specific for lymphocytes and/or bone marrow and/or polymorphonuclear leucocytes, have been used for the analysis of cells with identifiable membrane antigens. Populations that have cells with two of the above antigens in the membranes were identified. To these ends, complement-mediated cell kill by antisera alone and in mixtures was employed.

摘要

用于鉴定兔细胞标志物的试剂研发速度相对较慢。在本文中,兔细胞通过针对T细胞抗原(RTLA)、B细胞抗原(RABELA)和鼠类Ia抗原类似物的多克隆抗体进行表征。一些对淋巴细胞和/或骨髓和/或多形核白细胞具有特异性的单克隆抗体已被用于分析具有可识别膜抗原的细胞。鉴定出了膜上具有上述两种抗原的细胞群体。为此,单独使用抗血清以及混合使用抗血清介导补体杀伤细胞。

相似文献

1
Characterization of rabbit cells by monoclonal and polyclonal antibodies.用单克隆抗体和多克隆抗体对兔细胞进行鉴定。
Immunology. 1986 Sep;59(1):115-22.
2
Preparation and characterization of monoclonal antibodies recognizing three distinct differentiation antigens (BL1, BL2, BL3) on human B lymphocytes.识别人类B淋巴细胞上三种不同分化抗原(BL1、BL2、BL3)的单克隆抗体的制备与特性分析
J Immunol. 1984 Aug;133(2):684-91.
3
MALA-1: a surface antigen expressed on activated murine T and B lymphocytes.MALA-1:一种在活化的小鼠T淋巴细胞和B淋巴细胞上表达的表面抗原。
J Immunol. 1984 Jul;133(1):345-50.
4
Preparation and characterization of monoclonal antibodies reactive with porcine PBL.与猪外周血淋巴细胞反应的单克隆抗体的制备与鉴定
J Immunol. 1984 Jul;133(1):368-75.
5
Cell surface antigens expressed by subsets of pre-B cells and B cells.前B细胞和B细胞亚群表达的细胞表面抗原。
J Immunol. 1984 Jan;132(1):332-9.
6
Depletion of T cells from bone marrow for allogeneic transplantation: method for treatment of bone marrow in bulk.
Exp Hematol. 1986 Jan;14(1):21-6.
7
[Antibodies against human lymphocytes].
Gan To Kagaku Ryoho. 1982 Dec;9(12):2229-35.
8
In vitro treatment of human acute lymphocytic leukemia cells in bone marrow with a cocktail of monoclonal antibodies.用单克隆抗体混合物对骨髓中的人急性淋巴细胞白血病细胞进行体外处理。
Jpn J Clin Oncol. 1984 Dec;14 Suppl 1:537-42.
9
Expression of T cell differentiation antigens and Ia on rat cytotoxic T lymphocytes.大鼠细胞毒性T淋巴细胞上T细胞分化抗原和Ia的表达
J Immunol. 1982 Feb;128(2):580-4.
10
Reaction of various human normal and leukemic cells and cells of different cell lines with rabbit antisera prepared against membrane fraction of B and T cell lines.各种人类正常细胞、白血病细胞以及不同细胞系的细胞与针对B和T细胞系膜组分制备的兔抗血清的反应。
Neoplasma. 1983;30(2):187-95.

本文引用的文献

1
Interactions between rabbit polymorphonuclear leucocytes and staphylococci.兔多形核白细胞与葡萄球菌之间的相互作用。
J Exp Med. 1959 Sep 1;110(3):419-43. doi: 10.1084/jem.110.3.419.
2
Monoclonal antibodies to rabbit lymphoid cells: preparation and characterization of a T-cell-specific antibody.针对兔淋巴细胞的单克隆抗体:一种T细胞特异性抗体的制备与特性分析
Mol Immunol. 1981 Sep;18(9):815-22. doi: 10.1016/0161-5890(81)90003-1.
3
The persisting (P) cell: histamine content, regulation by a T cell-derived factor, origin from a bone marrow precursor, and relationship to mast cells.持久(P)细胞:组胺含量、受T细胞衍生因子的调节、起源于骨髓前体细胞以及与肥大细胞的关系。
Proc Natl Acad Sci U S A. 1981 Jan;78(1):323-7. doi: 10.1073/pnas.78.1.323.
4
In in vitro production and cloning of the P cell, a bone marrow-derived null cell that expresses H-2 and Ia-antigens, has mast cell-like granules, and is regulated by a factor released by activated T cells.在P细胞的体外生产和克隆过程中,P细胞是一种源自骨髓的无功能细胞,它表达H-2和Ia抗原,具有肥大细胞样颗粒,并受活化T细胞释放的一种因子调控。
J Immunol. 1981 Feb;126(2):452-8.
5
Growth of factor-dependent hemopoietic precursor cell lines.因子依赖性造血前体细胞系的生长
J Exp Med. 1980 Oct 1;152(4):1036-47. doi: 10.1084/jem.152.4.1036.
6
Granulocyte/macrophage-, megakaryocyte-, eosinophil- and erythroid-colony-stimulating factors produced by mouse spleen cells.小鼠脾细胞产生的粒细胞/巨噬细胞、巨核细胞、嗜酸性粒细胞和红细胞集落刺激因子。
Biochem J. 1980 Feb 1;185(2):301-14. doi: 10.1042/bj1850301.
7
P cell-stimulating factor: biochemical characterization of a new T cell-derived factor.P细胞刺激因子:一种新的T细胞衍生因子的生化特性
J Immunol. 1981 Nov;127(5):1941-7.
8
Rabbit leukocyte surface antigens defined by monoclonal antibodies.由单克隆抗体定义的兔白细胞表面抗原。
Eur J Immunol. 1983 Nov;13(11):919-28. doi: 10.1002/eji.1830131111.
9
Differentiation antigens identify subpopulations of rabbit T and B lymphocytes. Definition by flow cytometry.分化抗原可识别兔T淋巴细胞和B淋巴细胞的亚群。通过流式细胞术进行定义。
J Exp Med. 1983 Jan 1;157(1):34-46. doi: 10.1084/jem.157.1.34.
10
T cell antigens and MHC determinants on rabbit granulocytes.兔粒细胞上的T细胞抗原和主要组织相容性复合体决定簇
Immunol Lett. 1984;7(4):181-6. doi: 10.1016/0165-2478(84)90040-3.