Krensky A M, Clayberger C, Greenstein J L, Crimmins M, Burakoff S J
J Immunol. 1983 Dec;131(6):2777-80.
A human cytotoxic T lymphocyte (CTL) line, A9, was generated by limiting dilution and was selected because of its apparent DC specificity. A9 is 100% OKT3+, 90% OKT4+, and 10% OKT8+, but by negative selection the CTL present are entirely OKT8+. These OKT8+ CTL are totally inhibitable by Genox 3.53, an anti-DC1 monoclonal antibody (mAb), and Leu-10, an anti-DC subgroup mAb, but are not inhibitable by a panel of anti-HLA-DR mAb. These CTL are also inhibitable by anti-OKT3 and anti-LFA-2 but not by OKT4 or OKT8 mAb. These findings extend previous studies that showed that OKT8+ CTL recognize HLA-A,B antigens, whereas OKT4+ CTL recognize HLA-DR and SB antigens. It is possible that an as yet undefined T cell surface molecule is involved in DC recognition.
通过有限稀释法建立了一个人细胞毒性T淋巴细胞（CTL）系A9，因其明显的树突状细胞（DC）特异性而被挑选出来。A9细胞100%为OKT3阳性、90%为OKT4阳性、10%为OKT8阳性，但经过阴性选择后，现存的CTL完全为OKT8阳性。这些OKT8阳性CTL可被抗DC1单克隆抗体（mAb）Genox 3.53和抗DC亚群mAb Leu-10完全抑制，但不能被一组抗HLA-DR mAb抑制。这些CTL也可被抗OKT3和抗LFA-2抑制，但不能被OKT4或OKT8 mAb抑制。这些发现扩展了先前的研究，即OKT8阳性CTL识别HLA-A、B抗原，而OKT4阳性CTL识别HLA-DR和SB抗原。可能有一个尚未明确的T细胞表面分子参与DC识别。
