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使用蛋白质珠作为免疫吸附剂用于淋巴细胞的柱分级分离。

The use of protein beads as immunoadsorbent for the column fractionation of lymphocytes.

作者信息

Eckert R, von Broen B, Michael B, Jenssen H L, Köhler H J, Werner H

出版信息

Arch Immunol Ther Exp (Warsz). 1983;31(2):183-9.

PMID:6606411
Abstract

Beads of calf serum proteins (CSB) with antigen-antibody complexes or antigen by activation with glutaraldehyde were used as immunoadsorbents for the fractionation of mouse lymphocytes. T-lymphocytes could be separated by this method with high purity. The precursors of antibody-forming cells were completely eliminated from spleen and bone marrow cell suspensions. The unspecific binding of lymphocytes to CBS or Sepharose was similar and not selective for T or B lymphocytes.

摘要

用戊二醛活化的带有抗原-抗体复合物或抗原的小牛血清蛋白(CSB)珠作为免疫吸附剂用于小鼠淋巴细胞的分离。用这种方法可以高纯度分离出T淋巴细胞。抗体形成细胞的前体从脾细胞和骨髓细胞悬液中被完全清除。淋巴细胞与CBS或琼脂糖的非特异性结合相似,对T或B淋巴细胞没有选择性。

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