Herrod H G, Valenski W R
Clin Exp Immunol. 1981 Sep;45(3):562-7.
The formation of T lymphocyte colonies was studied in 30 normal subjects and 12 patients with suspected abnormalities in immune function. The mean number of colonies per plate in normal subjects was 1.159 +/- 411 which represented a plating efficiency of 0.5-1.0%. When individual cells from colonies of these normal subjects were studied for membrane markers, greater than 90% were E rosette-positive and less than 1% were positive for surface immunoglobulins. Blood lymphocytes obtained from all 12 patients showed diminished colony-forming capacity when compared to normal subjects with a range of 0-311 colonies. Six patients had less than 50 colonies/plate. Colony formation was diminished in some patients who had normal E rosette formation and lymphocyte proliferation in liquid culture. Because of these discrepancies it appears that colony formation is not a direct reflection of E rosette formation and lymphocyte proliferation. Evaluation of T lymphocyte colony-forming capacity may prove useful as an additional in vitro assessment of lymphocyte function.
对30名正常受试者和12名疑似免疫功能异常的患者的T淋巴细胞集落形成情况进行了研究。正常受试者每平板集落的平均数量为1.159±411，代表接种效率为0.5 - 1.0%。当对这些正常受试者集落中的单个细胞进行膜标志物研究时，超过90%为E花环阳性，表面免疫球蛋白阳性的细胞少于1%。与正常受试者相比，从所有12名患者获得的血淋巴细胞集落形成能力降低，集落数范围为0 - 311个。6名患者每平板集落数少于50个。一些在液体培养中E花环形成和淋巴细胞增殖正常的患者，其集落形成减少。由于这些差异，似乎集落形成并非E花环形成和淋巴细胞增殖的直接反映。评估T淋巴细胞集落形成能力可能作为淋巴细胞功能的一种额外体外评估方法而被证明是有用的。
