[Effect of uncouplers of oxidative phosphorylation on ATP concentration in frog striated muscle following changes in the buffer capacity of the solution].

Uncouplers of oxidative phosphorylation--gramicidin D, tetrachloro-2-trifluoromethylbenzimidasole (TTFB) and carbonylcyanide-m-trichlorphenylhydrazone (CCCP)--reduced ATP concentration in muscle. An increase of the buffer capacity of solution was without effect on uncoupling action of TTFB and CCCP but increased that of gramicidin D. The results can be explained by a different mechanism of the transfer of ion carriers through the membrane. It has been shown that ATP is not directly concerned with the membrane potential.