Laval F, Michel S
Cancer Lett. 1982 Jan;15(1):61-5. doi: 10.1016/0304-3835(82)90076-3.
The combined effects of hyperthermia and uncouplers of oxidative phosphorylation (dinitrophenol (DNP) or m-chlorocarbonylcyanide phenyl-hydrazone (CCCP) or 5-thio-d-glucose (5-TG) on mammalian cell survival were studied in vitro. Uncouplers were toxic towards cells treated under aerobic conditions at 41 degrees C, whereas 5-TG potentiated the effect of hyperthermia in the case of hypoxic cells. In aerobic conditions the intracellular ATP concentration was decreased upon action of uncouplers, and similar changes occurred in hypoxic cells treated with 5-TG. The results suggest that the ATP deprivation enhances the cell killing by hyperthermia.
在体外研究了热疗与氧化磷酸化解偶联剂（二硝基苯酚（DNP）、间氯羰基氰化物苯基腙（CCCP）或5-硫代-D-葡萄糖（5-TG））对哺乳动物细胞存活的联合作用。解偶联剂对在41℃有氧条件下处理的细胞有毒性，而在缺氧细胞中5-TG增强了热疗的效果。在有氧条件下，解偶联剂作用后细胞内ATP浓度降低，用5-TG处理的缺氧细胞也发生了类似变化。结果表明，ATP缺乏增强了热疗对细胞的杀伤作用。
