脆弱拟杆菌产生的新型β-内酰胺酶的特性
Properties of novel beta-lactamase produced by Bacteroides fragilis.
作者信息
Yotsuji A, Minami S, Inoue M, Mitsuhashi S
出版信息
Antimicrob Agents Chemother. 1983 Dec;24(6):925-9. doi: 10.1128/AAC.24.6.925.
Abstract
Bacteroides fragilis strains were isolated from clinical specimens. B. fragilis G-237 was highly resistant to beta-lactam antibiotics due to beta-lactamase production. The purified enzyme from this strain gave a single protein band on polyacrylamide gel electrophoresis. The isoelectric point was 4.8, and the molecular weight was estimated to be 26,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme activity was inhibited by p-chloromercuribenzoate and iodine but not by clavulanic acid or sulbactam. The purified enzyme showed a unique substrate profile by hydrolyzing at a high rate most of the cephalosporins, including cephamycin derivatives, penicillins, and imipenem (formerly imipemide, N-formimidoyl thienamycin, or MK 0787).
摘要
脆弱翻译为
脆弱拟杆菌菌株从临床标本中分离得到。脆弱拟杆菌G-237因产生β-内酰胺酶而对β-内酰胺类抗生素高度耐药。从该菌株纯化得到的酶在聚丙烯酰胺凝胶电泳上呈现单一蛋白条带。其等电点为4.8，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳估计分子量为26000。该酶的活性受到对氯汞苯甲酸和碘的抑制，但不受克拉维酸或舒巴坦的抑制。纯化后的酶通过高速水解大多数头孢菌素（包括头霉素衍生物、青霉素和亚胺培南（原亚胺培南、N-甲酰亚胺基硫霉素或MK 0787））显示出独特的底物谱。
相似文献
1
Properties of novel beta-lactamase produced by Bacteroides fragilis.脆弱拟杆菌产生的新型β-内酰胺酶的特性
Antimicrob Agents Chemother. 1983 Dec;24(6):925-9. doi: 10.1128/AAC.24.6.925.
2
Purification and characterization of an imipenem hydrolysing metallo-beta-lactamase from Bacteroides fragilis.脆弱拟杆菌中一种亚胺培南水解金属β-内酰胺酶的纯化与特性分析
J Antimicrob Chemother. 1992 Feb;29(2):105-13. doi: 10.1093/jac/29.2.105.
3
Properties of a new penicillinase type produced by Bacteroides fragilis.脆弱拟杆菌产生的一种新型青霉素酶的特性
Antimicrob Agents Chemother. 1982 Oct;22(4):579-84. doi: 10.1128/AAC.22.4.579.
4
Biochemical properties of beta-lactamase produced by Flavobacterium odoratum.芳香黄杆菌产生的β-内酰胺酶的生化特性
Antimicrob Agents Chemother. 1985 Apr;27(4):612-4. doi: 10.1128/AAC.27.4.612.
5
Beta-lactamases of type culture strains of the Bacteroides fragilis group and of strains that hydrolyse cefoxitin, latamoxef and imipenem.脆弱拟杆菌群典型培养菌株以及水解头孢西丁、拉氧头孢和亚胺培南的菌株的β-内酰胺酶
J Med Microbiol. 1986 Feb;21(1):49-57. doi: 10.1099/00222615-21-1-49.
6
Biochemical properties and purification of metallo-beta-lactamase from Bacteroides fragilis.脆弱拟杆菌金属β-内酰胺酶的生化特性及纯化
Antimicrob Agents Chemother. 1991 Feb;35(2):371-2. doi: 10.1128/AAC.35.2.371.
7
Purification and properties of inducible penicillin beta-lactamase isolated from Pseudomonas maltophilia.从嗜麦芽窄食单胞菌中分离出的诱导型青霉素β-内酰胺酶的纯化及特性
Antimicrob Agents Chemother. 1982 Oct;22(4):564-70. doi: 10.1128/AAC.22.4.564.
8
Characterization of cephalosporinases from Bacteroides fragilis, Bacteroides thetaiotaomicron and Bacteroides vulgatus.脆弱拟杆菌、多形拟杆菌和普通拟杆菌中头孢菌素酶的特性分析
J Antibiot (Tokyo). 1983 Jan;36(1):76-85. doi: 10.7164/antibiotics.36.76.
9
Studies on beta-lactamase in Bacteroides fragilis.脆弱拟杆菌中β-内酰胺酶的研究。
Infection. 1980;8 Suppl 2:S167-70. doi: 10.1007/BF01639885.
10
Purification and properties of a new beta-lactamase from Pseudomonas cepacia.洋葱伯克霍尔德菌新型β-内酰胺酶的纯化及性质
Antimicrob Agents Chemother. 1980 Mar;17(3):355-8. doi: 10.1128/AAC.17.3.355.
引用本文的文献
1
Epidemiology of β-Lactamase-Producing Pathogens.产β-内酰胺酶病原体的流行病学。
Clin Microbiol Rev. 2020 Feb 26;33(2). doi: 10.1128/CMR.00047-19. Print 2020 Mar 18.
2
Updated functional classification of beta-lactamases.β-内酰胺酶的功能分类更新。
Antimicrob Agents Chemother. 2010 Mar;54(3):969-76. doi: 10.1128/AAC.01009-09. Epub 2009 Dec 7.
3
Bacteroides fragilis group: trends in resistance.脆弱拟杆菌群：耐药性趋势
Curr Microbiol. 2006 Feb;52(2):153-7. doi: 10.1007/s00284-005-0249-x. Epub 2006 Jan 31.
4
Multicenter study of in vitro susceptibility of the Bacteroides fragilis group, 1995 to 1996, with comparison of resistance trends from 1990 to 1996.1995年至1996年脆弱拟杆菌群体外药敏的多中心研究，以及1990年至1996年耐药趋势比较
Antimicrob Agents Chemother. 1999 Oct;43(10):2417-22. doi: 10.1128/AAC.43.10.2417.
5
Rapid detection and evaluation of clinical characteristics of emerging multiple-drug-resistant gram-negative rods carrying the metallo-beta-lactamase gene blaIMP.携带金属β-内酰胺酶基因blaIMP的新型多重耐药革兰阴性杆菌临床特征的快速检测与评估
Antimicrob Agents Chemother. 1998 Aug;42(8):2006-11. doi: 10.1128/AAC.42.8.2006.
6
Oligonucleotide probes for detection of cephalosporinases among Bacteroides strains.用于检测拟杆菌属菌株中头孢菌素酶的寡核苷酸探针。
Antimicrob Agents Chemother. 1996 Apr;40(4):1014-6. doi: 10.1128/AAC.40.4.1014.
7
Multifocal outbreaks of metallo-beta-lactamase-producing Pseudomonas aeruginosa resistant to broad-spectrum beta-lactams, including carbapenems.对包括碳青霉烯类在内的广谱β-内酰胺耐药的产金属β-内酰胺酶铜绿假单胞菌的多灶性暴发。
Antimicrob Agents Chemother. 1996 Feb;40(2):349-53. doi: 10.1128/AAC.40.2.349.
8
beta-Lactamases in laboratory and clinical resistance.实验室及临床耐药中的β-内酰胺酶
Clin Microbiol Rev. 1995 Oct;8(4):557-84. doi: 10.1128/CMR.8.4.557.
9
In vitro antibacterial activity and beta-lactamase stability of a new carbapenem, BO-2727.新型碳青霉烯类药物BO - 2727的体外抗菌活性及β-内酰胺酶稳定性
Antimicrob Agents Chemother. 1995 Oct;39(10):2331-6. doi: 10.1128/AAC.39.10.2331.
10
A beta-lactamase from Serratia marcescens hydrolyzing the 2-carboxypenam T-5575.一种来自粘质沙雷氏菌的β-内酰胺酶，可水解2-羧基青霉烷T-5575。
Antimicrob Agents Chemother. 1995 Sep;39(9):2132-4. doi: 10.1128/AAC.39.9.2132.
本文引用的文献
1
In vitro and in vivo antibacterial activity of T-1982, a new semisynthetic cephamycin antibiotic.新型半合成头孢霉素抗生素T-1982的体外和体内抗菌活性
Antimicrob Agents Chemother. 1982 Nov;22(5):728-34. doi: 10.1128/AAC.22.5.728.
2
Properties of a new penicillinase type produced by Bacteroides fragilis.脆弱拟杆菌产生的一种新型青霉素酶的特性
Antimicrob Agents Chemother. 1982 Oct;22(4):579-84. doi: 10.1128/AAC.22.4.579.
3
Purification and properties of inducible penicillin beta-lactamase isolated from Pseudomonas maltophilia.从嗜麦芽窄食单胞菌中分离出的诱导型青霉素β-内酰胺酶的纯化及特性
Antimicrob Agents Chemother. 1982 Oct;22(4):564-70. doi: 10.1128/AAC.22.4.564.
4
Effect of N-formimidoyl thienamycin (MK0787) on beta-lactamases and activity against beta-lactamase-producing strains.N-甲脒基硫霉素（MK0787）对β-内酰胺酶的作用及其对产β-内酰胺酶菌株的活性。
Antimicrob Agents Chemother. 1980 Nov;18(5):837-8. doi: 10.1128/AAC.18.5.837.
5
Characterization of three different beta-lactamases from the Bacteroides fragilis group.脆弱拟杆菌群中三种不同β-内酰胺酶的特性分析。
Antimicrob Agents Chemother. 1980 Aug;18(2):220-5. doi: 10.1128/AAC.18.2.220.
6
Induction of beta-lactamase by various beta-lactam antibiotics in Enterobacter cloacae.多种β-内酰胺类抗生素对阴沟肠杆菌β-内酰胺酶的诱导作用。
Antimicrob Agents Chemother. 1980 Sep;18(3):382-5. doi: 10.1128/AAC.18.3.382.
7
Activity of beta-lactamase produced by Bacteroides fragilis against newly introduced cephalosporins.脆弱拟杆菌产生的β-内酰胺酶对新引入头孢菌素的活性。
Antimicrob Agents Chemother. 1980 Apr;17(4):736-7. doi: 10.1128/AAC.17.4.736.
8
Moxalactam (6059-S), a novel 1-oxa-beta-lactam with an expanded antibacterial spectrum: laboratory evaluation.莫西拉坦（6059-S），一种新型的具有扩大抗菌谱的1-氧杂-β-内酰胺：实验室评估
Antimicrob Agents Chemother. 1980 Mar;17(3):302-12. doi: 10.1128/AAC.17.3.302.
9
Characterization of cephalosporinases from Bacteroides fragilis, Bacteroides thetaiotaomicron and Bacteroides vulgatus.脆弱拟杆菌、多形拟杆菌和普通拟杆菌中头孢菌素酶的特性分析
J Antibiot (Tokyo). 1983 Jan;36(1):76-85. doi: 10.7164/antibiotics.36.76.
10
Bacteroides penicillinase.拟杆菌青霉素酶
J Bacteriol. 1968 Oct;96(4):1437-8. doi: 10.1128/jb.96.4.1437-1438.1968.
Zotero 插件