Holowczak J A, Joklik W K
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Virology. 1967 Dec;33(4):717-25. doi: 10.1016/0042-6822(67)90072-4.
Radioactively labeled vaccinia virions grown in L cells were dissociated to yield the constituent polypeptide chains, which were then subjected to electrophoresis in polyacrylamide gels. Mechanical fractionation of these gels yielded complex profiles in which at least 17 components, some major, some minor, could be reproducibly identified. The relative mass in each component has been calculated from the amount of radioactivity incorporated. The principal component (VSP-4) accounts for about 28% of the total viral protein mass. Cores derived from virions by chemical treatment contain the principal viral protein component, which is clearly multiple, as well as two minor ones (VSP-1 and VSP-2), which are probably single polypeptide species. These latter two components are the two slowest moving ones, and therefore most probably have the largest molecular weights. Treatment of virions with the nonionic detergent NP 40 liberates one major viral protein component (VSP-6). This component accounts for 18.7% of viral protein, the second highest amount, and is also multiple in nature.
在L细胞中培养的放射性标记痘苗病毒粒子被解离，以产生组成多肽链，然后将这些多肽链在聚丙烯酰胺凝胶中进行电泳。对这些凝胶进行机械分离得到复杂的图谱，其中至少可以重复鉴定出17种成分，有些是主要成分，有些是次要成分。已根据掺入的放射性量计算出每个成分的相对质量。主要成分（VSP-4）约占病毒总蛋白质量的28%。通过化学处理从病毒粒子中获得的核心含有主要的病毒蛋白成分，该成分显然是多种成分，还有两种次要成分（VSP-1和VSP-2），它们可能是单一多肽种类。后两种成分是迁移最慢的两种成分，因此最有可能具有最大的分子量。用非离子去污剂NP 40处理病毒粒子可释放出一种主要的病毒蛋白成分（VSP-6）。该成分占病毒蛋白的18.7%，含量第二高，并且本质上也是多种成分。
