Hume D A, Gordon S
Exp Cell Res. 1984 Feb;150(2):347-55. doi: 10.1016/0014-4827(84)90578-0.
Plasminogen activator activity and [3H]thymidine incorporation were studied in mouse bone marrow-derived macrophages. The two activities correlated closely in the presence of stimulatory (colony-stimulating factor, phorbol myristate acetate, PMA) and inhibitory (dexamethasone, prostaglandin E1) signals. The actions of dexamethasone and prostaglandin E1 could be overcome by either stimulatory agent, so that the net effect was an alteration in sensitivity of the macrophages to colony-stimulating factor, or PMA. The sensitivity of bone marrow-derived macrophages to CSF-1 was also reduced by the addition of small numbers of CSF-1 unresponsive peritoneal macrophages. Plasminogen activator induction was not a sufficient signal for [3H]thymidine incorporation which requires an additional macromolecular serum component. The serum component was found not to be plasminogen.
在小鼠骨髓来源的巨噬细胞中研究了纤溶酶原激活物活性和[3H]胸苷掺入情况。在存在刺激信号（集落刺激因子、佛波醇肉豆蔻酸酯乙酸酯，PMA）和抑制信号（地塞米松、前列腺素E1）时，这两种活性密切相关。地塞米松和前列腺素E1的作用可被任何一种刺激剂克服，因此净效应是巨噬细胞对集落刺激因子或PMA的敏感性改变。加入少量对CSF-1无反应的腹腔巨噬细胞也会降低骨髓来源巨噬细胞对CSF-1的敏感性。纤溶酶原激活物的诱导不是[3H]胸苷掺入的充分信号，[3H]胸苷掺入需要另一种大分子血清成分。发现该血清成分不是纤溶酶原。
