Flesch I, Ferber E
Immunobiology. 1986 Mar;171(1-2):14-26. doi: 10.1016/S0171-2985(86)80014-6.
A serum-free medium for mass production of pure murine bone marrow-derived macrophages in liquid culture was developed. Iscove's modified Dulbecco's medium (IMDM) was used as a basal nutrient medium supplemented with 2-mercaptoethanol, transferrin and a source of colony-stimulating factor (CSF). Addition of exogenous lipids or bovine serum albumin was not required. The macrophages can be kept viable for at least eight days in the serum-free culture medium, and they are able to incorporate tritiated thymidine. The tritiated thymidine incorporation can be enhanced by retinoic acid, phorbol myristate acetate (PMA), colony-stimulating factor (CSF) and transferrin. Prostaglandin E1, prostaglandin E2 and dexamethasone inhibited tritiated thymidine incorporation. The serum-free culture of bone marrow-derived macrophages has some important implications. First, signal molecules can be defined which enhance or inhibit the proliferative capacity of bone marrow-derived macrophages. Second, the fatty acid composition of membrane phospholipids can be altered to study the relationship between macrophage function and membrane lipid composition.
开发了一种用于在液体培养中大规模生产纯小鼠骨髓来源巨噬细胞的无血清培养基。Iscove改良的杜尔贝科培养基（IMDM）用作基础营养培养基，并添加了2-巯基乙醇、转铁蛋白和集落刺激因子（CSF）来源。不需要添加外源脂质或牛血清白蛋白。巨噬细胞在无血清培养基中可存活至少八天，并且它们能够掺入氚标记的胸腺嘧啶核苷。维甲酸、佛波醇肉豆蔻酸酯乙酸盐（PMA）、集落刺激因子（CSF）和转铁蛋白可增强氚标记的胸腺嘧啶核苷掺入。前列腺素E1、前列腺素E2和地塞米松抑制氚标记的胸腺嘧啶核苷掺入。骨髓来源巨噬细胞的无血清培养具有一些重要意义。首先，可以确定增强或抑制骨髓来源巨噬细胞增殖能力的信号分子。其次，可以改变膜磷脂的脂肪酸组成以研究巨噬细胞功能与膜脂质组成之间的关系。
