Boersma W J
Exp Hematol. 1983 Nov;11(10):922-30.
Mouse bone marrow prothymocytes as determined in an in vivo thymus regeneration assay have an in vitro gamma radiation sensitivity which is different from that of spleen colony forming cells (CFUs). Determination of Do according to in vivo irradiation revealed similar but insignificant differences. Prothymocytes in normal bone marrow maintain a low but slightly different proliferative state as compared to CFUs, according to determinations using the 3H-TdR suicide technique. In regenerating bone marrow prothymocytes were found to be sensitive to an inhibitory effect of in vitro incubation with cold thymidine. CFUs and normal bone marrow prothymocytes were not affected by cold thymidine. Taking into account the cold thymidine effect it can be concluded that prothymocytes and CFUs in regenerating bone marrow are fully in cycle. These results are best explained when prothymocytes and CFUs are considered to be different cells.
在体内胸腺再生试验中所测定的小鼠骨髓前胸腺细胞，其体外γ射线敏感性与脾集落形成细胞（CFU）不同。根据体内照射测定Do显示出相似但不显著的差异。根据使用³H-胸腺嘧啶核苷自杀技术的测定，正常骨髓中的前胸腺细胞与CFU相比维持着较低但略有不同的增殖状态。在再生骨髓中，发现前胸腺细胞对与冷胸腺嘧啶核苷进行体外孵育的抑制作用敏感。CFU和正常骨髓前胸腺细胞不受冷胸腺嘧啶核苷的影响。考虑到冷胸腺嘧啶核苷的作用，可以得出结论，再生骨髓中的前胸腺细胞和CFU完全处于细胞周期中。当认为前胸腺细胞和CFU是不同细胞时，这些结果能得到最好的解释。
