Matutes E, Robinson D, O'Brien M, Haynes B F, Zola H, Catovsky D
Leuk Res. 1983;7(6):787-801. doi: 10.1016/0145-2126(83)90073-5.
The ultrastructural and immunologic features of normal convoluted T-lymphocytes were studied with a panel of monoclonal antibodies and the immunogold technique and these were compared with cells from patients with Sézary syndrome (SS) and adult T-cell lymphoma-leukaemia (ATLL). According to the characteristics of the nucleus, two distinct T-cell subtypes, representing 2-4% of normal peripheral blood lymphocytes were recognized: (i) a cerebriform lymphocyte ('Sézary-like') characterized by narrow and deep nuclear indentations, closely resembling the cells of SS, and (ii) a convoluted or polylobated lymphocyte ('ATLL-like'), with shorter and broader nuclear indentations than those seen in SS, that resemble the cells of ATLL. Both types of lymphocytes were positive with the monoclonal antibodies OKT3, OKT4, OKT17 and FMC3 and were negative with OKT8, OKM1 and FMC4 (anti-HLA-Dr) as SS and ATLL cells. A difference was observed with the expression of the anti-T monoclonal antibody 3Al: Sézary-like lymphocytes, like SS and ATLL cells, were 3Al negative whilst ATLL-like lymphocytes were 3Al positive. The close morphological and membrane phenotype similarities observed between these two types of lymphocytes and the cells from SS and ATLL suggest that they may well represent the normal counterparts of the malignant T cells in both conditions.
运用一组单克隆抗体和免疫金技术研究了正常曲核T淋巴细胞的超微结构和免疫特征，并将其与蕈样肉芽肿综合征（SS）和成人T细胞淋巴瘤白血病（ATLL）患者的细胞进行了比较。根据细胞核的特征，识别出了两种不同的T细胞亚型，占正常外周血淋巴细胞的2%-4%：（i）脑回状淋巴细胞（“类蕈样肉芽肿细胞”），其特征为核凹陷狭窄且深，与SS细胞极为相似；（ii）曲核或多叶状淋巴细胞（“类成人T细胞淋巴瘤白血病细胞”），其核凹陷比SS细胞中的更短更宽，与ATLL细胞相似。这两种淋巴细胞与单克隆抗体OKT3、OKT4、OKT17和FMC3反应呈阳性，与OKT8、OKM1和FMC4（抗HLA-Dr）反应呈阴性，与SS和ATLL细胞相同。在抗T单克隆抗体3Al的表达上观察到差异：类蕈样肉芽肿细胞淋巴细胞与SS和ATLL细胞一样，3Al呈阴性，而类成人T细胞淋巴瘤白血病细胞淋巴细胞3Al呈阳性。这两种淋巴细胞与SS和ATLL细胞之间在形态和膜表型上的密切相似性表明，它们很可能分别代表这两种疾病中恶性T细胞的正常对应物。
