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通过免疫结合鉴定支原体菌落。

Identification of mycoplasma colonies by immunobinding.

作者信息

Kotani H, McGarrity G J

出版信息

J Clin Microbiol. 1986 Apr;23(4):783-5. doi: 10.1128/jcm.23.4.783-785.1986.

DOI:10.1128/jcm.23.4.783-785.1986
PMID:3084556
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC362837/
Abstract

An immunobinding assay was developed to identify mycoplasma colonies on agar in pure and mixed cultures. Mycoplasma colonies on agar were transferred to nitrocellulose. The nitrocellulose was treated with specific rabbit antisera against mycoplasmas, peroxidase-conjugated goat anti-rabbit immunoglobulin G, 4-chloro-1-naphthol, and H2O2. Purple developed in the presence of specific reactions. The procedure was superior to epifluorescence.

摘要

开发了一种免疫结合试验，以鉴定纯培养物和混合培养物中琼脂上的支原体菌落。将琼脂上的支原体菌落转移到硝酸纤维素膜上。硝酸纤维素膜用针对支原体的特异性兔抗血清、过氧化物酶偶联的山羊抗兔免疫球蛋白G、4-氯-1-萘酚和H2O2处理。在特异性反应存在的情况下会出现紫色。该方法优于落射荧光法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58e9/362837/aa5b04150511/jcm00240-0122-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58e9/362837/e88a85a96497/jcm00240-0122-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58e9/362837/2f3116384ab9/jcm00240-0122-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58e9/362837/aa5b04150511/jcm00240-0122-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58e9/362837/e88a85a96497/jcm00240-0122-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58e9/362837/2f3116384ab9/jcm00240-0122-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58e9/362837/aa5b04150511/jcm00240-0122-c.jpg

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本文引用的文献

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MYCOPLASMA SPECIES IDENTIFICATION BASED UPON GROWTH INHIBITION BY SPECIFIC ANTISERA.基于特异性抗血清生长抑制的支原体种类鉴定
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Rapid detection of Bordetella pertussis by a monoclonal antibody-based colony blot assay.基于单克隆抗体的菌落印迹法快速检测百日咳博德特氏菌
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