通过免疫结合鉴定支原体菌落。

Kotani H, McGarrity G J

J Clin Microbiol. 1986 Apr;23(4):783-5. doi: 10.1128/jcm.23.4.783-785.1986.

An immunobinding assay was developed to identify mycoplasma colonies on agar in pure and mixed cultures. Mycoplasma colonies on agar were transferred to nitrocellulose. The nitrocellulose was treated with specific rabbit antisera against mycoplasmas, peroxidase-conjugated goat anti-rabbit immunoglobulin G, 4-chloro-1-naphthol, and H2O2. Purple developed in the presence of specific reactions. The procedure was superior to epifluorescence.

开发了一种免疫结合试验，以鉴定纯培养物和混合培养物中琼脂上的支原体菌落。将琼脂上的支原体菌落转移到硝酸纤维素膜上。硝酸纤维素膜用针对支原体的特异性兔抗血清、过氧化物酶偶联的山羊抗兔免疫球蛋白G、4-氯-1-萘酚和H2O2处理。在特异性反应存在的情况下会出现紫色。该方法优于落射荧光法。