H-Y抗原的放射免疫测定
Radioimmunoassay for H-Y antigen.
作者信息
Savikurki H, Pajunen L, Nemlander A, Andersson L C, De la Chapelle A
出版信息
J Immunol Methods. 1981;41(3):343-9. doi: 10.1016/0022-1759(81)90196-4.
PMID:7229387
Abstract
A sensitive direct radioimmunoassay for the detection of H-Y antigen on human blood mononuclear leukocytes is described. The cells were incubated with antisera obtained by immunization of inbred female rats or mice with male cells and tissues. The bound antibodies were detected by 125I-labeled protein A. Results are expressed as cpm bound to the cells.
摘要
本文描述了一种用于检测人血单核白细胞上H-Y抗原的灵敏直接放射免疫测定法。将细胞与用雄性细胞和组织免疫近交系雌性大鼠或小鼠获得的抗血清一起孵育。通过125I标记的蛋白A检测结合的抗体。结果以与细胞结合的每分钟计数（cpm）表示。
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