Ikezawa Z, Nagy Z A, Klein J
J Immunol. 1984 Apr;132(4):1605-7.
Hybridomas produced by fusion between the BW5147 thymoma and an LDH-B-specific B10.A(2R) suppressor T cell line secrete two T suppressor factors (TsF). One factor (TsF-A) shares Mhc determinants with the A alpha A beta molecule and suppresses proliferating Th cells; the other (TsF-E) shares determinants with the E alpha E beta molecule and it inhibits the maturation of the T suppressor (Ts) cells. Here we demonstrate that the two factors can be used to alter the immune response status of cultured T lymphocytes or of an animal. When added to a culture of LDH-B-primed cells or injected into mice, the TsF-A turns responders into nonresponders, presumably by blocking the proliferation of the Th cells. The TsF-E converts nonresponder cultures or mice into responders, presumably by preventing the differentiation of Ts cells. As there are good prospects for obtaining TsF in large quantities and in a highly purified form, this manipulation of the immune response by the deployment of specific factors promises to become an efficient new method of immunotherapy.
由BW5147胸腺瘤与乳酸脱氢酶B特异性B10.A(2R)抑制性T细胞系融合产生的杂交瘤分泌两种T抑制因子（TsF）。一种因子（TsF-A）与AαAβ分子共享Mhc决定簇，并抑制增殖的Th细胞；另一种（TsF-E）与EαEβ分子共享决定簇，并抑制T抑制（Ts）细胞的成熟。在此我们证明，这两种因子可用于改变培养的T淋巴细胞或动物的免疫反应状态。当添加到乳酸脱氢酶B致敏细胞培养物中或注射到小鼠体内时，TsF-A将反应者转变为无反应者，推测是通过阻断Th细胞的增殖。TsF-E将无反应者培养物或小鼠转变为反应者，推测是通过阻止Ts细胞的分化。由于大量获得高纯度TsF的前景良好，通过部署特定因子对免疫反应进行这种操纵有望成为一种有效的新免疫治疗方法。
