Sadamori N, Block A W, Han T, Sandberg A A
Cancer Genet Cytogenet. 1984 Apr;11(4):395-8. doi: 10.1016/0165-4608(84)90019-0.
To clarify the cell cycle duration of stimulated cells in B cell chronic lymphocytic leukemia (B-CLL), sister chromatid differentiation (SCD) methodology was utilized. So-called polyclonal B-cell activators (PBA), i.e., staphylococcus bacteria strain Cowan I (Cowan I), pokeweed mitogen (PWM), Epstein-Barr virus (EBV), and lipopolysaccharide W from E. coli 055:B5 (LPS), were examined. Most metaphases on day 2 (48 hr) of culture were in first division (M1), and about half of the metaphases on day 3 (72 hr) of culture were in the second division (M2), and many of the metaphases on day 4 (96 hr) of culture were in the third division. These facts suggest that the optimal culture time for cytogenetic study of B-CLL should be 3 days or less to avoid in vitro artifacts.
为了阐明B细胞慢性淋巴细胞白血病（B-CLL）中受刺激细胞的细胞周期持续时间，采用了姐妹染色单体分化（SCD）方法。研究了所谓的多克隆B细胞激活剂（PBA），即考恩I型葡萄球菌菌株（Cowan I）、商陆有丝分裂原（PWM）、爱泼斯坦-巴尔病毒（EBV）和来自大肠杆菌055:B5的脂多糖W（LPS）。培养第2天（48小时）的大多数中期细胞处于第一次分裂（M1），培养第3天（72小时）约一半的中期细胞处于第二次分裂（M2），培养第4天（96小时）许多中期细胞处于第三次分裂。这些事实表明，B-CLL细胞遗传学研究的最佳培养时间应为3天或更短，以避免体外假象。
