Hoffman R A, Kung P C, Hansen W P, Goldstein G
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4914-7. doi: 10.1073/pnas.77.8.4914.
A simple and rapid method for the determination of human T lymphocyte subclasses in buffy coat preparations or whole blood is described. This technique uses flow cytometry to distinguish lymphocytes from other leukocytes on the basis of their light-scattering properties. Lymnphocyte subclasses were enumerated by cellular immunofluorescence; the immunofluorescent signals were produced by monoclonal antibodies to surface differentiation antigens on T cells. Conventional techniques of enumerating T lymphocyte subclasses entail time-consuming (up to 2 days) density gradient and E rosette enrichement, and require at least 20 ml of blood. The method described here uses as little as 50 microliters of whole blood for each antibody tested and produces results within in 2 hr.
本文描述了一种用于测定血沉棕黄层制剂或全血中人类T淋巴细胞亚群的简单快速方法。该技术利用流式细胞术，根据淋巴细胞的光散射特性将其与其他白细胞区分开来。通过细胞免疫荧光法对淋巴细胞亚群进行计数；免疫荧光信号由针对T细胞表面分化抗原的单克隆抗体产生。传统的T淋巴细胞亚群计数技术需要耗时（长达2天）的密度梯度和E花环富集，且至少需要20毫升血液。此处所述方法每次检测抗体仅需50微升全血，并可在2小时内得出结果。
