Durrant L G, Parkar M, Kenworthy N, Taylor G M
Immunology. 1984 May;52(1):117-28.
Human lymphocytes stimulated with phytohaemagglutinin (PHA) were fused with an HGPRT- murine lymphoma, BW5147, and a hybridoma BwFc93-1 was isolated and cloned in agarose. This human X mouse hybrid and nine clones derived from it were characterized by chromosome analysis, phenotypic and functional assays. Karyotyping and isoenzyme studies showed the presence of five human chromosomes in BwFc93-1 with preferential retention of three chromosomes--6, X and 15--in the clones. Membrane immunofluorescence analysis revealed that all the clones expressed human and mouse class 1 MHC antigens and the mouse T cell antigens Thy-1 and T200, but were devoid of human OKT3, OKT8 and mouse Lyt-2. Human OKT4 and OKM1 phenotypes were transiently expressed by one clone and mouse Lyt 1 by two other clones. Several T4-, Lyt-1- clones produced and bound human interleukin-2 (IL-2) indicating a lack of correlation between human T cell phenotype and function in those hybrids. There was also evidence of dichotomy in the secretion of IL-2 and expression of the IL-2 receptor since clones were identified which either bound or secreted IL-2. One clone expressing IL-2 receptors could be induced to produce human IL-2 by simultaneously stimulating with PHA and phorbol myristate acetate (PMA).
用植物血凝素（PHA）刺激的人淋巴细胞与一种次黄嘌呤鸟嘌呤磷酸核糖转移酶缺陷型（HGPRT-）小鼠淋巴瘤细胞系BW5147进行融合，分离得到杂交瘤BwFc93-1并在琼脂糖中进行克隆。通过染色体分析、表型和功能测定对这个人鼠杂交细胞及其九个克隆进行了特征鉴定。核型分析和同工酶研究表明，BwFc93-1中存在五条人类染色体，其克隆中优先保留了三条染色体——6号、X染色体和15号染色体。膜免疫荧光分析显示，所有克隆均表达人类和小鼠的Ⅰ类主要组织相容性复合体（MHC）抗原以及小鼠T细胞抗原Thy-1和T200，但不表达人类OKT3、OKT8和小鼠Lyt-2。一个克隆短暂表达人类OKT4和OKM1表型，另外两个克隆短暂表达小鼠Lyt 1表型。几个T4-、Lyt-1-克隆产生并结合人类白细胞介素-2（IL-2），表明在这些杂交细胞中人T细胞表型与功能之间缺乏相关性。此外，还存在IL-2分泌和IL-2受体表达二分法的证据，因为鉴定出了要么结合要么分泌IL-2的克隆。一个表达IL-2受体的克隆可以通过同时用PHA和佛波酯（PMA）刺激而被诱导产生人类IL-2。
