DeFreitas E C, Vella S, Linnenbach A, Zmijewski C, Koprowski H, Croce C M
Proc Natl Acad Sci U S A. 1982 Nov;79(21):6646-50. doi: 10.1073/pnas.79.21.6646.
Human T cell hybridomas were produced by fusing the hypoxanthine phosphoribosyltransferase-deficient line of the human T cell lymphoma Jurkat with a continuous line of normal human T cells specific for tetanus toxoid (TeT). The hybridomas were selected for their ability to produce interleukin 2 after exposure to TeT on semiautologous monocytes and for their ability to bind to TeT-pulsed semiautologous monocytes. These antigen-specific T hybridomas demonstrated potent helper activity for semiautologous B cells as determined by the production of high levels of anti-TeT antibody in vitro.
通过将人T细胞淋巴瘤Jurkat的次黄嘌呤磷酸核糖基转移酶缺陷型细胞系与针对破伤风类毒素(TeT)的正常人T细胞连续系融合,制备了人T细胞杂交瘤。这些杂交瘤因在半自体单核细胞暴露于TeT后产生白细胞介素2的能力以及与TeT脉冲半自体单核细胞结合的能力而被筛选出来。如通过体外产生高水平抗TeT抗体所测定的,这些抗原特异性T杂交瘤对半自体B细胞表现出强大的辅助活性。