Sly W S, Sekhon G S, Kennett R, Bodmer W F, Bodmer J
Tissue Antigens. 1976 Mar;7(3):165-72. doi: 10.1111/j.1399-0039.1976.tb01047.x.
Permanent human lymphoid cell lines were established successfully from peripheral blood lymphocytes which had been separated for HL-A typing and stored in liquid nitrogen for two years. Frozen lymphocytes were chosen from two siblings who were homozygous at the LA and FOUR HL-A loci. Thawed lymphocytes were transformed with EB virus produced by the marmoset lymphoid line B95-8. No chromosome abnormalities were seen on karyotypes prepared on cells from the established human lymphoid lines using G and Q banding techniques. HL-A typing showed the expected HL-A antigens plus a considerable number of additional reactions. Separation of lymphocytes and freezing them for possible future use requires a relatively small investment. This method of preserving cells can be applied to patients with interesting genetic disorders or other biochemical markers to provide cells which can be transformed and propagated years later.
成功地从为进行HL - A分型而分离并在液氮中保存了两年的外周血淋巴细胞中建立了永久性人类淋巴细胞系。从在LA和四个HL - A位点纯合的两个同胞中选取冷冻淋巴细胞。解冻的淋巴细胞用狨猴淋巴细胞系B95 - 8产生的EB病毒进行转化。使用G和Q显带技术对建立的人类淋巴细胞系的细胞制备的核型进行分析，未发现染色体异常。HL - A分型显示出预期的HL - A抗原以及大量额外的反应。分离淋巴细胞并将其冷冻以备将来可能使用所需的投资相对较小。这种保存细胞的方法可应用于患有有趣的遗传疾病或其他生化标记的患者，以提供数年后可转化和增殖的细胞。
