Kumar S, Jasani B, Hunt J S, Moffat D B, Asscher A W
Histochem J. 1985 Nov;17(11):1251-8. doi: 10.1007/BF01002506.
The distribution of Tamm-Horsfall protein (THP) was studied in the human kidney using formalin-fixed, paraffin-embedded sections with a monoclonal antibody specific for human THP applied in conjunction with a modified dinitrophenyl hapten sandwich staining (DHSS) procedure. The method was found to be highly sensitive producing very strong specific staining at antibody dilutions up to 1 in 64 000. Counterstaining with Haematoxylin and Eosin was possible without significant masking of the specific staining. This provided excellent structural definition of the background tissue which proved especially important in the study of THP localization in randomly oriented biopsy material. THP was found in all segments of the thick ascending limbs of loops of Henle, most segments of distal convoluted tubules and occasionally in distended collecting ducts and in the glomerular capsular space. Maculae densae did not contain THP. The combination of the modified DHSS procedure and the human THP specific antibody represents a highly sensitive and reliable method for specific staining of the THP in kidney sections.
利用福尔马林固定、石蜡包埋切片，结合应用针对人Tamm-Horsfall蛋白（THP）的单克隆抗体和改良的二硝基苯基半抗原夹心染色（DHSS）方法，研究了THP在人肾脏中的分布。结果发现该方法高度灵敏，在抗体稀释至1:64000时仍能产生非常强的特异性染色。用苏木精和伊红复染是可行的，且不会显著掩盖特异性染色。这为背景组织提供了出色的结构清晰度，这在随机取向的活检材料中THP定位研究中尤为重要。在髓袢升支粗段的所有节段、远曲小管的大多数节段中均发现了THP，偶尔在扩张的集合管和肾小球囊腔中也有发现。致密斑不含THP。改良的DHSS方法与针对人THP的特异性抗体相结合，是一种对肾脏切片中THP进行特异性染色的高度灵敏且可靠的方法。
