Beck H P, Schneider E M, Wallenfels B, Lipps H J, Schott H, Sonneborn H, Wernet P
Cell Biol Int Rep. 1984 Mar;8(3):193-205. doi: 10.1016/0309-1651(84)90031-6.
Interleukin 2 (IL 2) is a lymphokine with specific growth promoting properties for activated T cells. The isolation and purification of specific mRNAs coding for this protein is reported. The analysis of total poly A + containing RNA from PHA stimulated T lymphocytes on agarose gels reveals the existence of at least two different IL-2 mRNAs. Isolated RNAs have been microinjected separately into Xenopus laevis oocytes for translation and consecutively the synthesized proteins were tested for activity on alloactivated T cell lines which were dependent on IL-2 for growth. Both RNAs coded for a protein which displayed clearly detectable IL-2 activity.
