Parker J, Caldini G, Krishnamurti C, Ahrens P B, Ankel H
FEBS Lett. 1984 May 21;170(2):391-5. doi: 10.1016/0014-5793(84)81351-4.
Exogenous gangliosides inhibit interleukin 2 (IL2)-dependent growth of a T cell line, AKIL -1.E8. IL2 activity is retained by columns of ganglioside covalently linked to poly(L-lysine)-agarose and is not eluted with ethylene glycol but is completely recovered by elution with 1% SDS. The ability of gangliosides to inhibit IL2 activity is directly related to the complexity of their carbohydrate portion, and related ceramide derivatives at similar concentrations do not inhibit IL2 activity. We conclude that IL2 bound to exogenous gangliosides is inactive and that the carbohydrate portion of the ganglioside is crucial to its interaction with IL2.
外源性神经节苷脂可抑制T细胞系AKIL -1.E8依赖白细胞介素2（IL2）的生长。IL2活性可被共价连接到聚（L-赖氨酸）-琼脂糖上的神经节苷脂柱保留，不能用乙二醇洗脱，但用1%十二烷基硫酸钠洗脱可完全回收。神经节苷脂抑制IL2活性的能力与其碳水化合物部分的复杂性直接相关，类似浓度的相关神经酰胺衍生物不抑制IL2活性。我们得出结论，与外源性神经节苷脂结合的IL2无活性，神经节苷脂的碳水化合物部分对其与IL2的相互作用至关重要。
