Jensen C, Svendsen U G, Thastrup O, Stahl Skov P, Leon A, Norn S
Agents Actions. 1987 Jun;21(1-2):79-82. doi: 10.1007/BF01974925.
The influence of exogenous addition of gangliosides on histamine release from human basophils and rat mast cells was examined in vitro. Gangliosides dose-dependently inhibited histamine release, and this inhibition was dependent on the ganglioside sialic acid content, since GT1b, having 3 sialic acid moieties, was more potent than gangliosides with 2 moieties (GD1a and GD1b), which again were more potent inhibitors than GM1 with one moiety. Asialo-GM1 was without effect. In high concentrations the gangliosides potentiated basophil histamine release. The modulation of histamine release was reflected in the sensitivity of the cells to extracellular calcium, since inhibition of the release could be counteracted by increasing the extracellular concentration of calcium.
体外研究了外源性添加神经节苷脂对人嗜碱性粒细胞和大鼠肥大细胞组胺释放的影响。神经节苷脂呈剂量依赖性抑制组胺释放，且这种抑制作用取决于神经节苷脂的唾液酸含量，因为含有3个唾液酸部分的GT1b比含有2个部分的神经节苷脂（GD1a和GD1b）更有效，而GD1a和GD1b又比含有1个部分的GM1是更有效的抑制剂。脱唾液酸GM1没有作用。在高浓度下，神经节苷脂增强嗜碱性粒细胞组胺释放。组胺释放的调节反映在细胞对细胞外钙的敏感性上，因为增加细胞外钙浓度可抵消释放的抑制作用。
