Rifas L, Shen V, Mitchell K, Peck W A
Proc Natl Acad Sci U S A. 1984 Jul;81(14):4558-62. doi: 10.1073/pnas.81.14.4558.
Rat resident peritoneal macrophages in primary culture were found to elaborate a mitogenic factor (or factors) for rat osteoblast-like cells and chondrocytes but not for skin fibroblasts. Growth-promoting activity appeared in the incubation medium within the first 20 hr of macrophage culture and was released in amounts that paralleled the number of macrophages per culture. After their proliferative response, as judged by increases in DNA synthesis and cell number, the osteoblast-like cells became enriched in alkaline phosphatase, an index of osteoblast specialization. The macrophage-derived activity was nondialyzable and heat-stable, and it was eliminated by exposure to trypsin. Inhibition of prostaglandin cyclooxygenase failed to modify its generation. Partial purification (Amicon filter concentration, gel filtration) disclosed principal peaks of activity corresponding to Mr of 43,000 and 10,000. The crude conditioned medium and the Mr 43,000-peak, but not the low-molecular-weight peak, exhibited interleukin 1 activity, as judged by the ability to stimulate the proliferation of mouse thymic lymphocytes. The macrophage-derived growth factor described herein may participate in bone remodeling and repair and in primary bone and cartilage growth.
原代培养的大鼠腹腔巨噬细胞可产生一种对大鼠成骨样细胞和软骨细胞有促有丝分裂作用的因子（或多种因子），但对皮肤成纤维细胞无此作用。在巨噬细胞培养的最初20小时内，促生长活性出现在培养液中，其释放量与每培养物中的巨噬细胞数量成正比。经DNA合成增加和细胞数量增多判断，成骨样细胞在增殖反应后，碱性磷酸酶含量增加，碱性磷酸酶是成骨细胞特化的一个指标。巨噬细胞衍生的活性物质不能透析且对热稳定，经胰蛋白酶处理后活性消失。抑制前列腺素环氧化酶并不能改变其产生。部分纯化（Amicon滤器浓缩、凝胶过滤）显示主要活性峰对应的分子量为43,000和10,000。根据刺激小鼠胸腺淋巴细胞增殖的能力判断，粗制条件培养液和分子量43,000的峰具有白细胞介素1活性，而低分子量峰则没有。本文所述的巨噬细胞衍生生长因子可能参与骨重塑和修复以及原发性骨和软骨生长。
