Bensouyad A, Hollander A P, Dularay B, Bedwell A E, Cooper R A, Hutton C W, Dieppe P A, Elson C J
Department of Pathology, Bristol University.
Ann Rheum Dis. 1990 May;49(5):301-7. doi: 10.1136/ard.49.5.301.
The dimethylmethylene blue assay showed higher concentrations of glycosaminoglycans in many synovial fluids from patients with rheumatoid arthritis (RA) than in autologous sera or sera or synovial fluids from normal subjects. These results were taken to suggest that the glycosaminoglycans in RA synovial fluid were abnormally raised and derived from cartilage. To discover what stimulated such glycosaminoglycan release in RA joints relations were sought between synovial fluid concentrations of glycosaminoglycans and immunological and inflammatory mediators. It was shown that RA synovial fluid glycosaminoglycan concentrations correlated with synovial fluid C3d concentrations but not with synovial fluid rheumatoid factor concentrations, polymorphonuclear leucocyte numbers, myeloperoxidase concentrations, or the ability of the synovial fluids to release free radicals from normal polymorphonuclear leucocytes. A correlation was found between synovial fluid C3d and interleukin 1 concentrations as judged by both lymphocyte activating factor activity and immunoassay, but no significant correlation was detected between interleukin 1 and glycosaminoglycan concentrations. It is suggested that in the rheumatoid joint locally produced cytokines, in addition to interleukin 1, together stimulate glycosaminoglycan release from cartilage and render it vulnerable to attack by other processes.
二甲亚甲基蓝检测显示，类风湿性关节炎（RA）患者的许多滑液中糖胺聚糖的浓度高于自体血清或正常受试者的血清及滑液中的浓度。这些结果表明，RA滑液中的糖胺聚糖异常升高且源自软骨。为了探究是什么刺激了RA关节中这种糖胺聚糖的释放，研究了滑液中糖胺聚糖浓度与免疫和炎症介质之间的关系。结果显示，RA滑液中糖胺聚糖浓度与滑液C3d浓度相关，但与滑液类风湿因子浓度、多形核白细胞数量、髓过氧化物酶浓度或滑液从正常多形核白细胞释放自由基的能力无关。通过淋巴细胞激活因子活性和免疫测定判断，滑液C3d与白细胞介素1浓度之间存在相关性，但白细胞介素1与糖胺聚糖浓度之间未检测到显著相关性。有人提出，在类风湿关节中，除白细胞介素1外，局部产生的细胞因子共同刺激软骨释放糖胺聚糖，并使其易受其他过程的攻击。
