Popp-Snijders C, Lomecky M Z, de Jong A P
Clin Chim Acta. 1983 Jul 31;132(1):83-9. doi: 10.1016/0009-8981(83)90235-8.
A gas-liquid chromatographic method, using a fused silica capillary column, for the determination of red cell sorbitol is described. The capillary column gives complete resolution of the polyols xylitol, inositol, mannitol, sorbitol and galactitol, even when the glucose peak in the red cell chromatogram is dominating. The identity of sorbitol, and its single elution from the capillary column has been confirmed by mass spectrometry. Recovery of sorbitol from various red cell samples was 101% +/- 3.2 (mean +/- SD, n = 7). Precision, estimated from duplicate diabetic red cell sorbitol analyses was CVdup = 3.5% (n = 18) and from run to run analyses CVinterassay = 4.0% (n = 6). Sorbitol levels determined in erythrocytes of 19 healthy subjects were 5.9 +/- 1.6 nmol/ml red cells and in erythrocytes of 18 insulin-dependent diabetics 17.8 +/- 8.2 nmol/ml red cells (means +/- SD). The method described offers a reliable and specific tool to study in vivo polyol pathway activity in relation to some diabetes-associated complications.
本文描述了一种使用熔融石英毛细管柱的气-液色谱法，用于测定红细胞山梨醇。即使红细胞色谱图中的葡萄糖峰占主导地位，该毛细管柱也能完全分离多元醇木糖醇、肌醇、甘露醇、山梨醇和半乳糖醇。山梨醇的同一性及其从毛细管柱的单一洗脱已通过质谱法得到证实。从各种红细胞样品中回收山梨醇的回收率为101%±3.2（平均值±标准差，n = 7）。根据糖尿病患者红细胞山梨醇重复分析估计的精密度为CVdup = 3.5%（n = 18），批间分析的CVinterassay = 4.0%（n = 6）。19名健康受试者红细胞中山梨醇水平为5.9±1.6 nmol/ml红细胞，18名胰岛素依赖型糖尿病患者红细胞中山梨醇水平为17.8±8.2 nmol/ml红细胞（平均值±标准差）。所描述的方法为研究与某些糖尿病相关并发症有关的体内多元醇途径活性提供了一种可靠且特异的工具。
