Rapid analysis of sorbitol, galactitol, mannitol and myoinositol mixtures from biological sources.

The three commonly found hexitols mannitol, sorbitol and galactitol are well separated from each other and from myoinositol by gas chromatography as their butylboronate derivative on Dexsil-400, on a 1:1 mixture of OV-1 and OV-17, or on a DB-17 fused-silica capillary column. The method allows all four substances to be measured by autosampling electron ionization gas chromatography-mass spectrometry (GC-MS) in small tissue samples at organ concentrations as small as 5 mumol/kg wet mass in less than 4 min. Comparisons were made to determine the relative sensitivity of GC-MS and other detection methods. The order of sensitivity was electron ionization GC-MS greater than chemical ionization GC-MS greater than flame photometric detection using a boron-selective filter greater than hydrogen flame ionization detection.