Hargis J R
J Exp Zool. 1976 Apr;196(1):39-44. doi: 10.1002/jez.1401960105.
Differences between ventilatory response and metabolic rates of young rainbow trout tested within the sublethal range of pH 6 to pH 9 were observed using a flowing water respirometer. The oxygen consumption was monitored at swimming speeds of 12 cm/sec and 24 cm/sec. The oxygen consumption rates at 24 cm/sec and pH 6 (423 mg/kg-hr) and pH 9 (367 mg/kg-hr) were considerably higher than those determined near neutrality (328 mg/kg-hr). Ventilation rate increased to either side of neutrality, but significantly fewer respiratory reversals, or "coughs," were observed at pH 6 and a greater number at pH 9 than occurred at pH 7 and 8 or in untested fish. The respiratory-cough response is shown to be pH-dependent in rainbow trout and may therefore not be as reliable an indication of pollutant-caused stress in studies where the experimental pH has not been specified or controlled.
使用流水式呼吸计观察了在pH值6至pH值9的亚致死范围内测试的幼年虹鳟鱼的通气反应和代谢率之间的差异。在12厘米/秒和24厘米/秒的游泳速度下监测氧气消耗量。在24厘米/秒的游泳速度下，pH值6（423毫克/千克-小时）和pH值9（367毫克/千克-小时）时的氧气消耗率明显高于接近中性时测定的消耗率（328毫克/千克-小时）。通气率在中性值两侧均增加，但在pH值6时观察到的呼吸反转或“咳嗽”次数明显少于pH值7和8时或未测试的鱼，而在pH值9时观察到的次数更多。结果表明，虹鳟鱼的呼吸-咳嗽反应依赖于pH值，因此在未指定或控制实验pH值的研究中，它可能不是污染物引起的应激的可靠指标。
